---
title: My samples failed QC1, what should I do?
description: A QC1 failure can be a result of multiple things, each with its own unique resolution. Find out what may be causing your QC1 failure.
---

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# My samples failed QC1, what should I do?

## A QC1 failure can be a result of multiple things, each with its own unique resolution. Find out what may be causing your QC1 failure.

First, double-check your **Proximally Ligated DNA** yield. The Arima HiC protocol has a maximum input of 5ug. If your Proximally Ligated DNA yield was over 5ug, try lowering your input. 

If your yield is in the range of **500ng-5ug**, we can look into other areas to improve your QC1 score. There are 2 incubation times that often improve QC1 scores.

1. Increase **Conditioning** from 10 minutes to 20 minutes
2. Increase **Digestion** at 37C from 60 minutes to overnight

### What does QC1 measure and why is it important?

QC1 measures the percentage of DNA that has been biotinylated from the biotin fill-in step. A passing QC1 score is **greater than 15%**.

QC1 scores correlate with how well the downstream data analysis will look and processing a simple with a failing QC1 will often yield unusable data.

 

If the adjustments in incubation times still do not result in improved QC1 scores, please contact tech support at **techsupport@arimagenomics.com**

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